- Details
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We measure vitamin K1 and K2 menaquinones in plasma or serum, in tissues andin food products. For our vitamin K measurement we are participating in the world wide KEQAS (vitamin K External Quality Assurance Scheme at www.keqas.com) to work according to the best standard and deliver quality to our customers. We also measure biochemical markers related to bone and cardiovascular metabolism.
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Biomarkers in serum or plasma.
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Measurement |
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Procedure |
Volume |
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(µL or gram) |
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Vitamin K |
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A |
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> 500 |
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Vitamin K |
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B |
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> 1000 |
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Vitamin D |
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A |
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> 60 |
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Total Osteocalcin |
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A |
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> 110 |
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Carboxylated Osteocalcin |
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A |
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> 250 |
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Uncarboxylated Osteocalcin |
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A |
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> 250 |
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Bone-specific alkaline phosphatase (BAB) |
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A |
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> 100 |
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N-terminal collagen crosslinks (NTX) |
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A |
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> 150 |
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Total -uncarboxylated MGP (tucMGP) |
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A / C |
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> 100 |
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Desphospho-uncarboxylated MGP (dpucMPG) |
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C |
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> 150 |
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desphospho-carboxylated MGP (dpucMPG) |
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C |
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> 100 |
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Procedure A |
1 Clothing of Full blood for 1/2 hour at room temperature. |
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2. Centrifugate at 3000x g (~4000 rpm). for 15 minutes at room temperature |
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g (~4000 rpm). |
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3. Transfer serum to a new vial (600 µL/vial) and stored at -80°C. |
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It is important to protect the samples from UV light! |
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Procedure B |
Use fresh food and transport at 4°C to Maastricht. |
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Procedure C |
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1. Collect full blood in citrated plasma (3.2%) |
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2. Centrifugate at 3000 times g (~4000rpm).room temperature for 15 minutes |
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3. Transfer plasmato a new vial (600 µL/vial) |
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and store at -80°C until transfer. |
